| Autor: |
A V, Yakhyayev, I M, Voronkova, V A, Sukhanov |
| Rok vydání: |
1991 |
| Předmět: |
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| Zdroj: |
Electrophoresis. 12(9) |
| ISSN: |
0173-0835 |
| Popis: |
A method is described for isoelectric focusing of proteins, using an ultrathin-layer polyacrylamide gel on cellophane, followed by electrophoretic transfer of separated proteins onto a nitrocellulose membrane. The polyacrylamide gel is firmly attached to the cellophane and thus protected from mechanical damage; such gels are easily manipulated. Cellophane is permeable to ions and application of this gel support overcomes difficulties resulting from the removal of ultrathin gels from a plastic support on electroblotting. Proteins separated under nondenaturing conditions were transferred onto a nitrocellulose membrane and detected by the concanavalin A-peroxidase technique. The proposed approach makes it possible to analyze the variability of nondenatured proteins and glycoproteins of different origin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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