| Autor: |
Cheryl V, Rahman, Dror, Ben-David, Amritpaul, Dhillon, Gisela, Kuhn, Toby W A, Gould, Ralph, Müller, Felicity R A J, Rose, Kevin M, Shakesheff, Erella, Livne |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Journal of tissue engineering and regenerative medicine. 8(1) |
| ISSN: |
1932-7005 |
| Popis: |
Sustained and controlled delivery of growth factors, such as bone morphogenetic protein 2 (BMP-2), from polymer scaffolds has excellent potential for enhancing bone regeneration. The present study investigated the use of novel sintered polymer scaffolds prepared using temperature-sensitive PLGA/PEG particles. Growth factors can be incorporated into these scaffolds by mixing the reconstituted growth factor with the particles prior to sintering. The ability of the PLGA/PEG scaffolds to deliver BMP-2 in a controlled and sustained manner was assessed and the osteogenic potential of these scaffolds was determined in a mouse calvarial defect model. BMP-2 was released from the scaffolds in vitro over 3 weeks. On average, ca. 70% of the BMP-2 loaded into the scaffolds was released by the end of this time period. The released BMP-2 was shown to be active and to induce osteogenesis when used in a cell culture assay. A substantial increase in new bone volume of 55% was observed in a mouse calvarial defect model for BMP-2-loaded PLGA/PEG scaffolds compared to empty defect controls. An increase in new bone volume of 31% was observed for PLGA/PEG scaffolds without BMP-2, compared to empty defect controls. These results demonstrate the potential of novel PLGA/PEG scaffolds for sustained BMP-2 delivery for bone-regeneration applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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