Autocrine TGF-beta-regulated expression of adhesion receptors and integrin-linked kinase in HT-144 melanoma cells correlates with their metastatic phenotype
| Autor: | B, Janji, C, Melchior, V, Gouon, L, Vallar, N, Kieffer |
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| Rok vydání: | 1999 |
| Předmět: |
Reverse Transcriptase Polymerase Chain Reaction
Integrin beta1 Blotting Western Integrin beta3 Down-Regulation Platelet Membrane Glycoproteins Protein Serine-Threonine Kinases Cadherins Flow Cytometry Up-Regulation Phenotype Matrix Metalloproteinase 9 Antigens CD Cell Movement Transforming Growth Factor beta Tumor Cells Cultured Humans Collagenases Melanoma Cell Size |
| Zdroj: | International journal of cancer. 83(2) |
| ISSN: | 0020-7136 |
| Popis: | We have previously shown that 2 human melanoma cell lines, the metastatic HT-144 and the non-metastatic SK-Mel-2 cells, exhibit marked in vitro heterogeneity with respect to integrin expression, migration and invasion potential. Here, we provide evidence that HT-144 melanoma cells, but not SK-Mel-2 cells, undergo a reversible transition to a fibroblastoid morphology following treatment with either their own serum-free acidified conditioned medium or biologically active exogenous TGF-beta1, thus identifying TGF-beta as an autocrine regulator of the spindle shape morphology of HT-144 melanoma cells. The fibroblastoid phenotype correlated with up-regulated beta1 and beta3 integrin and down-regulated E-cadherin expression, as shown by flow cytometry, Western blot and RT-PCR, as well as up-regulated expression of the matrix metalloproteinase MMP-9, as demonstrated by zymography. Our data further illustrate the TGF-beta1-dependent up-regulation of integrin-linked kinase and the nuclear translocation of beta-catenin, 2 intracellular proteins involved in integrin and cadherin signaling. |
| Databáze: | OpenAIRE |
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