Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3' MAR

Autor:	S Luke, Mankin, George C, Allen, Thomas, Phelan, Steven, Spiker, William F, Thompson
Rok vydání:	2003
Předmět:	Hot Temperature Genes Plant Plants Genetically Modified Polymerase Chain Reaction Up-Regulation Plant Leaves Transformation Genetic Gene Expression Regulation Plant Tobacco Nuclear Matrix Transgenes Promoter Regions Genetic 3' Untranslated Regions DNA Primers Glucuronidase
Zdroj:	Transgenic research. 12(1)
ISSN:	0962-8819
Popis:	We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promoters. Expression from an induced heat shock promoter was also increased and MARs did not cause expression in the absence of heat shock. There was also no effect of MARs on the pea ferredoxin promoter, which is not normally expressed in this cell line. Importantly, most transgenes flanked by RB7 MAR elements showed a large reduction in the number of low expressing GUS transformants relative to control constructs without MARs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::70a1d8f9c4efa47a3cca0bb703a3e5a7 https://pubmed.ncbi.nlm.nih.gov/12650520 Zobrazit plný text záznamu Full text from SpringerLink