Unmodified calcium concentrations in tumour necrosis factor receptor subtype-mediated apoptotic cell death

Autor:	S M, McFarlane, H M, Anderson, S J, Tucker, O J, Jupp, D J, MacEwan
Rok vydání:	2000
Předmět:	Time Factors Ionophores Cell Survival Tumor Necrosis Factor-alpha Ionomycin Apoptosis Receptors Tumor Necrosis Factor Cell Line Microscopy Fluorescence Antigens CD Rhabdomyosarcoma Humans Receptors Tumor Necrosis Factor Type II Calcium Fura-2 HeLa Cells
Zdroj:	Molecular and cellular biochemistry. 211(1-2)
ISSN:	0300-8177
Popis:	Tumour necrosis factor-alpha (TNF) receptors mediate a variety of effects dependent on cell type. A role for Ca2+ in TNF-induced death remains uncertain. Here we investigated restricting intracellular/extracellular Ca2+ in HeLa epithelial carcinoma cells expressing low and high levels of p75TNFR receptor subtype and KYM-1 rhabdomyosarcoma cells, models of rapid TNF-induced apoptosis. Ca2+ -chelators EGTA and BAPTA-AM as well as microsomal Ca2+ -ATPase inhibitor thapsigargin, did not alter TNF-induced death. TNF was also unable to alter resting [Ca2+]i levels which remained200 nM even during times when these cells were undergoing apoptotic cell death. These findings indicate no role for modulated Ca2+ concentrations in TNF-induced apoptotic cell death.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::7060bfb54454dd1560b6d28b7693f465 https://pubmed.ncbi.nlm.nih.gov/11055543 Zobrazit plný text záznamu Full text from SpringerLink