| Autor: |
K H, Oshima, T W, Comans, A K, Highsmith, E W, Ades |
| Rok vydání: |
1995 |
| Předmět: |
|
| Zdroj: |
Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association. 8(1) |
| ISSN: |
1077-9450 |
| Popis: |
We tested the ability of a 0.04-micron nylon membrane filter to remove human immunodeficiency virus (HIV) from tissue culture media containing 10% fetal calf serum. Endpoint titrations of infectious virus (ID50) were performed on lymphocyte cultures. The presence of virus in the cultures was determined using an enzyme-linked immunoabsorbant assay (ELISA) of the HIV-1 P24 core antigen. In repeated experiments, filtration of the virus suspension resulted in the removal of HIV below detectable limits. The titer reduction was estimated to be greater than 8.5 x 10(2). These results suggest that this filter is effective in removing HIV from fluids containing serum or serum products. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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