Binding thermodynamics of paromomycin, neomycin, neomycin-dinucleotide and -diPNA conjugates to bacterial and human rRNA

Autor:	Javier, Alguacil, Jordi, Robles, Clara, Ràfols, Elisabeth, Bosch
Rok vydání:	2014
Předmět:	RNA Bacterial Aminoglycosides Binding Sites Paromomycin RNA Ribosomal Humans Thermodynamics Neomycin Calorimetry Anti-Bacterial Agents Protein Binding
Zdroj:	Journal of molecular recognition : JMR. 29(4)
ISSN:	1099-1352
Popis:	Isothermal titration calorimetry (ITC) is a powerful technique able to evaluate the energetics of target-drug binding within the context of drug discovery. In this work, the interactions of RNAs reproducing bacterial and human ribosomal A-site, with two well-known antibiotic aminoglycosides, Paromomycin and Neomycin, as well as several Neomycin-dinucleotide and -diPNA conjugates, have been evaluated by ITC and the corresponding thermodynamic quantities determined. The comparison of the thermodynamic data of aminoglycosides and their chemical analogues allowed to select Neomycin-diPNA conjugates as the best candidates for antimicrobial activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::703545f8515c4e1aa8c09e7b1ddd7c89 https://pubmed.ncbi.nlm.nih.gov/26530431 Zobrazit plný text záznamu Plný text