| Autor: |
Chao-Long, Rao, Zheng-Chang, Heng |
| Rok vydání: |
2005 |
| Předmět: |
|
| Zdroj: |
Wei sheng yan jiu = Journal of hygiene research. 34(3) |
| ISSN: |
1000-8020 |
| Popis: |
Applying RDPCR to detect the DNA damage of N-ras gene induced by Potassium dichromatePreparing single-stranded probes of exon 1 of N-ras gene in human. The genomic DNA was treated with Potassium dichromate, then amplified by RDPCR and detected by Southern hybridization with the probe.The clear hybridized bands can be seen in the position which is induced by Potassium dichromate on the dose of 100 micromol/L, but can't be detected on the dose over 1000 mol/L.It indicates that Potassium dichromate can cause the DNA damage of N-ras gene, which should be the key point of its carcinogenesis mechanisms. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
