Induction of Endoplasmic Reticulum Stress via Reactive Oxygen Species Mediated by Luteolin in Melanoma Cells
| Autor: | Joon Ki, Kim, Kyoung Ah, Kang, Yea Seong, Ryu, Mei Jing, Piao, Xia, Han, Min Chang, Oh, Sun Jin, Boo, Seung Uk, Jeong, Yong Joo, Jeong, Sungwook, Chae, Soo-Young, Na, Jin Won, Hyun |
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| Rok vydání: | 2016 |
| Předmět: |
Staining and Labeling
Apoptosis Endoplasmic Reticulum Stress Transfection Activating Transcription Factor 6 Mitochondria Neoplasm Proteins Gene Expression Regulation Neoplastic Cell Line Tumor Humans Calcium RNA Small Interfering Luteolin Reactive Oxygen Species Melanoma Transcription Factor CHOP Tumor Stem Cell Assay |
| Zdroj: | Anticancer research. 36(5) |
| ISSN: | 1791-7530 |
| Popis: | This study aimed to investigate whether luteolin, a flavonoid, induces apoptosis in human melanoma cells via endoplasmic reticulum (ER) stress.To investigate the effects of luteolin in human melanoma cells, the anti-proliferation, apoptosis, ER stress induction and reactive oxygen species (ROS) generation were evaluated using MTT, Hoechst 33342, ER-tracker Blue White DPX and DCF-DA staining assays, respectively.Luteolin inhibited cell proliferation and increased apoptotic body formation. Luteolin induced ER stress, as shown by ER staining and mitochondrial Ca(2+) overloading. Luteolin increased expression of the ER stress-related proteins; protein kinase RNA-like ER kinase, phospho eukaryotic translation initiation factor 2α, activating transcription factor (ATF) 6, CCAAT/enhancer-binding protein-homologous protein (CHOP), and cleaved caspase 12. Furthermore, luteolin increased the level of intracellular ROS, leading to ROS-mediated apoptosis and ER stress. However, N-acetyl cysteine, a ROS scavenger, decreased ROS levels, apoptosis, and ER stress induced by luteolin treatment. In addition, knockdown of CHOP and ATF6 by small-interfering RNA inhibited luteolin-induced cell death.Luteolin induces apoptosis by ER stress via increasing ROS levels. |
| Databáze: | OpenAIRE |
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