| Autor: |
Lauren M, Smith, Conrad B, Pitts, Lanette J, Friesen-Waldner, Neetin H, Prabhu, Katherine E, Mathers, Kevin J, Sinclair, Trevor P, Wade, Timothy R H, Regnault, Charles A, McKenzie |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
J Magn Reson Imaging |
| ISSN: |
1522-2586 |
| Popis: |
BACKGROUND: Alterations in glycolysis are central to the increasing incidence of non-alcoholic fatty liver disease (NAFLD), highlighting a need for in vivo, non-invasive technologies to understand the development of hepatic metabolic aberrations. PURPOSE: To use hyperpolarized magnetic resonance spectroscopy (MRS) and proton density fat fraction (PDFF) MRI techniques to investigate the effects of a chronic, life-long exposure to the Western Diet (WD) in an animal model resulting in NAFLD; to investigate the hypothesis that exposure to the WD will result in NAFLD in association with altered pyruvate metabolism. STUDY TYPE: Prospective ANIMAL MODEL: 28 male guinea pigs weaned onto a control diet (n = 14) or WD (n = 14). FIELD STRENGTH/SEQUENCE: 3T; T1-weighted gradient echo, T2-weighted spin echo, 3D gradient multi-echo fat-water separation (IDEAL-IQ) and broadband point-resolved spectroscopy (PRESS) chemical-shift sequences. ASSESSMENT: Median PDFF was calculated in the liver and hind limbs. [1-(13)C]pyruvate dynamic MRS in the liver was quantified by the time to peak (TTP) for each metabolite. Animals were euthanized and tissue was analyzed for lipid and cholesterol concentration and enzyme level and activity. STATISTICAL TESTS: Unpaired Student’s t-tests were used to determine differences in measurements between the two diet groups. The Pearson correlation coefficient was calculated to determine correlations between measurements. RESULTS: Life-long WD consumption resulted in significantly higher liver PDFF and elevated triglyceride content in the liver. The WD group exhibited a decreased TTP for lactate production, and ex vivo analysis highlighted increased liver lactate dehydrogenase (LDH) activity. DATA CONCLUSION: PDFF MRI results suggest differential fat deposition patterns occurring in animals fed a life-long WD characteristic of lean, or lacking excessive subcutaneous fat, NAFLD. The decreased liver lactate TTP and increased ex vivo LDH activity suggest lipid accumulation occurs in association with a shift from oxidative metabolism to anaerobic glycolytic metabolism in WD-exposed livers. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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