A versatile ultra-high performance LC-MS method for lipid profiling
| Autor: | Oskar L, Knittelfelder, Bernd P, Weberhofer, Thomas O, Eichmann, Sepp D, Kohlwein, Gerald N, Rechberger |
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| Rok vydání: | 2013 |
| Předmět: |
WT
wild type CL cardiolipin Ultra-performance liquid chromatography Cer ceramide Glycerophospholipids PE phosphatidylethanolamine PG phosphatidylglycerol Mass Spectrometry Article PI phosphatidylinositol TIC total ion current Mice PC phosphatidylcholine Yeasts GP glycerophospholipid SE steryl ester DG diacylglycerol Animals Phosphoric Acids PA phosphatidic acid Chromatography High Pressure Liquid SM sphingomyelin ESI electrospray ionization Muscles MM minimal media PS phosphatidylserine Lipids TG triacylglycerol XIC extracted ion chromatogram NL neutral lipid Lipidomics lipids (amino acids peptides and proteins) C/M chloroform/methanol (2/1 v/v) BMP bis(monoacylglycero)phosphate Neutral lipids |
| Zdroj: | Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences |
| ISSN: | 1873-376X |
| Popis: | Highlights • UPLC-MSE based method for lipid separation and identification. • The method is suitable for polar and non-polar lipid species. • Excellent separation of lipid species within lipid classes. • Identification of low abundant lipid species. • May be combined with ESI-MS both in positive and negative ionization mode. A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. MSE scans allow simultaneous acquisition of full scan data and collision induced fragmentation to improve identification of lipid classes and to obtain structural information. The method was used to investigate the lipidome of yeast. |
| Databáze: | OpenAIRE |
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