| Autor: |
O V, Krasilnikov, P G, Merzlyak, L N, Yuldasheva, R A, Nogueira |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
General physiology and biophysics. 17(4) |
| ISSN: |
0231-5882 |
| Popis: |
The possibility of obtaining information about the radius of high and low conductance states of channels in multichannel membranes was tested experimentally. In spite of the interference of non-electrolytes on the numbers of channels that appeared in the membrane, the non-electrolyte-exclusion method was successfully adapted to multichannel bilayers to estimate the radius of the larger opening of the low conductance state of the channel induced by Staphylococcus aureus alpha-toxin. At the pH used, the channel transition to a low conductance state was accompanied by a decrease of the opening radius from 1.3 +/- 0.2 nm to 0.9 +/- 0.1 nm. The determination criteria for maximum size of a channel opening when using the non-electrolyte exclusion method is discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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