| Autor: |
Huandi, Ding, Ying, Han, Angen, Huang, Yuan, Meng, Huaxin, Hu, Xiaotong, Li |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 30(12) |
| ISSN: |
1007-8738 |
| Popis: |
To prepare and characterize the monoclonal antibody (mAb) against Helicobacter pylori (Hp) and establish a competitive ELISA used for detection of Hp antibodies in the sera of Hp-infected patients.BALB/c mice were immunized with inactivated Hp to generate Hp mAb using the hybridoma technology. Hp mixed proteins including cytotoxin-associated gene A (CagA), vacuolating cytotoxin A (VacA) and urease, as well as inactivated Hp were applied to screen positive hybridoma. Selected Hp mAb was analyzed and characterized with ELISA and Western blotting, and then labeled with horseradish peroxidase (HRP) for establishing a competitive ELISA to detect Hp antibodies in the sera of Hp-infected patients.One Hp mAb named as C3 was selected after screening large amount of hybridoma, and the C3 Hp mAb was special for IgG2a subtype with the affinity titer of 1×10(7). Western blotting, ELISA and mass spectrum analysis indicated that the C3 Hp mAb could recognize Hp urease subunit B specifically. Using the C3 Hp mAb, we developed a competitive ELISA which could be used to detect Hp antibodies in the sera of Hp-infected patients.We successfully obtained one mAb that could specifically recognize Hp urease subunit B and developed a competitive ELISA using the Hp mAb. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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