[Direct analysis of the frequency of disomy in human sperm using the PRINS technique]
| Autor: | I, Quenesson, A, Girardet, L, Coignet, B, Andréo, G, Lefort, J P, Charlieu, F, Pellestor |
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| Jazyk: | francouzština |
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Chromosomes Human Pair 12 Base Sequence Chromosomes Human Pair 21 Molecular Sequence Data Biotin DNA Satellite Polymerase Chain Reaction Spermatozoa Meiosis Microscopy Fluorescence Nondisjunction Genetic Humans Chromosomes Human Pair 9 Digoxigenin Chromosomes Human Pair 16 In Situ Hybridization Fluorescence DNA Primers |
| Zdroj: | Annales de genetique. 38(2) |
| ISSN: | 0003-3995 |
| Popis: | The PRINS method allows a rapid and specific detection of human chromosomes in situ. We have adapted the PRINS protocol to human sperm. Estimates of disomy have thus been performed for chromosomes 9, 12, 16 and 21 by using alpha-satellite DNA specific primers. The frequencies of disomy ranged from 0.27% to 0.31%. No significant difference was found. These data agree with the hypothesis of an equal distribution of non-disjunctions among chromosomes in male meiosis. |
| Databáze: | OpenAIRE |
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