| Autor: |
J K, Li, B K, Moloney, J L, Shupe, E J, Gardner, N C, Leone, Y, Elsner |
| Rok vydání: |
1989 |
| Předmět: |
|
| Zdroj: |
American journal of veterinary research. 50(6) |
| ISSN: |
0002-9645 |
| Popis: |
Genomic DNA polymorphisms obtained by restriction fragment-length polymorphism from healthy horses and horses with hereditary multiple exostoses were analyzed. These DNA were digested by 12 restriction enzymes and were hybridized against 6 isotopically labeled oncogene probes. Hybridization was not detected with the viral oncogene, v-ras, which indicated this oncogene was absent in the equine genome. Oncogenes (c-raf-1, c-fes, c-myb, c-myc, and c-sis) were present and had similar hybridization patterns and signal intensities in DNA from healthy horses and horses with hereditary multiple exostoses. Unique and distinct restriction fragment-length polymorphisms were detected with the c-raf-1 probe only in BamHI- and PstI-digested equine DNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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