Transfection of osteoclasts and osteoclast precursors

Autor: Julie C, Crockett, David J, Mellis, Adam, Taylor
Rok vydání: 2011
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 816
ISSN: 1940-6029
Popis: Osteoclasts and their precursors have traditionally been considered difficult cells to transfect using standard approaches. Here, we describe several methods for transfection of mature osteoclasts and their precursors using the Amaxa™ Nucleofector system, lentiviruses, and adenoviruses.
Databáze: OpenAIRE