| Autor: |
F A, Bettelheim, M B, Reid, D, Garland |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Experimental eye research. 58(2) |
| ISSN: |
0014-4835 |
| Popis: |
Four fractions of gamma-crystallins, B(II), C(IIIb), D(IIIa) and E(IVa), were isolated from bovine lenses. The hydration properties of each fraction were measured as a function of protein concentration. The freezable water content was obtained by differential scanning calorimetry and the total water content by thermogravimetric analysis. The difference yields the non-freezable (bound) water content of the crystallins. At physiological protein concentrations the order of the bound water content was C(IIIb)D(IIIa)E(IVa)B(II). This order was reversed between C and D and between B and E at low protein concentrations. This trend was evident whether the bound water was calculated as a percentage of the total water or as quantity per gram of gamma-crystallin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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