| Autor: |
Elba Veronica, Arias-Rios, Kristina, Tenney, Tam, Mai, Sam, Anderson, Ruth Marie, Cantera, Jasmine M, Pando, Brandon, Selover, Lourdes M, Nadala, Seana K, Davidson, Mansour, Samadpour |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Journal of AOAC International. 102(4) |
| ISSN: |
1944-7922 |
| Popis: |
BACKGROUND: Dairy products are common sources of Listeria outbreaks, and early detection of the pathogen is critical to prevent outbreaks of illnesses and financial losses for dairy producers. OBJECTIVE: This study aimed to evaluate Sample6 Detect HT/L for effective detection of Listeria monocytogenes and L. innocua in ice cream. METHODS: Performance of the Sample6 DETECT HT/L was compared with U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) Chapter 10 method for detection of Listeria spp. in ice cream using an unpaired study design. RESULTS: R2-enriched samples tested with Sample6 Detect HT/L performed as well as the reference method at all time points tested from 15 to 24 h. R2 is a proprietary blend for use with the test kit that helps with early detection. All the dPOD(C) values (Sample6 Detect HT/L presumptive and confirmed results) equaled zero, indicating 100% concordance between the methods. Both Sample6 Detect HT/L and FDA BAM results showed low dPOD(C) values, with confidence intervals indicating no significant differences between Sample6 Detect HT/L and reference method results. CONCLUSIONS: Sample6 Detect HT/L is suitable to detect Listeria spp. in ice cream, even with a 12 h enrichment. Sample6 Detect HT/L demonstrated equivalent detection of L. monocytogenes and L. innocua from R2-enriched samples as expected with 15 and 18 h enrichment when compared with the 24 h FDA BAM method for L. monocytogenes. HIGHLIGHTS: These results indicate that Sample6 Detect HT/L, primarily developed for environmental samples, can be used to detect Listeria spp. in ice cream with less incubation time, resulting in faster detection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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