| Popis: |
To investigate the effects of different routes in placental mesenchymal stem cells (PMSC) on serum expression levels of IL-4, IL-17, TNF-α and IFN-γ in aplastic anemia (AA) rats.The rat model of aplastic anemia (AA rats) was established by 5-fluorouracil combined with busulfan. The rats was divided into four groups: control, experimental, PMSC-injected into the tail vein, and PMSC-injected into the medullary cavity. The general state of rats in each group was observed in detail before and after treatment. The serum levels of interleukin-4 (IL-4) , interleukin-17 (IL-17), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) were measured by enzyme-linked immunosorbent assay (ELISA) at week 1, 3 and 5 after treatment.The serum levels of TNF-α, IFN-γ and IL-17 in the experimental group were significantly higher than those in the control group, while the level of IL-4 was significantly decreased (P＜0.05). The levels of TNF-α, IFN-γ and IL-17 gradually decreased after treatment while the level of IL-4 increased. By the fifth week, the above indexes were closed to the control group (P＞0.05), and the levels of TNF-α, IFN-γ and IL-17 in the group with PMSCs injected via the medullary cavity decrease more significantly than those group with PMSC injected via the tail vein, but level of IL-4 was not significantly different between two groups.The level of serum hematopoietic negative regulators increase significantly, and the level of hematopoietic promoting factors decreases significantly in aplastic anemia rats. PMSC can down-regulate the level of hematopoietic negative regulators and up-regulate the level of hematopoietic promoting factors in the rats with aplastic anemia, and the inhibition of hematopoietic negative regulators by intramedullary injection is more significant than that by caudal vein injection.不同途径输注PMSC对再生障碍性贫血鼠造血调控因子水平的影响.探讨不同途径输注的胎盘间充质干细胞（placental mesenchymal stem cell，PMSC）对再生障碍性贫血（aplastic anemia，AA）大鼠血清IL-4、IL-17、TNF-α、IFN-γ表达水平的影响.采用5-氟尿嘧啶联合白消安建立再生障碍性贫血大鼠模型（AA大鼠）；大鼠分为对照组、实验组、尾静脉注射PMSC组、髓腔注射PMSC组；治疗前后详细观察各组大鼠一般状态；以酶联免疫吸附试验（ELISA）检测治疗后1、3和5周各组大鼠血清白介素-4（IL-4）、白介素-17（IL-17）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）水平.实验组血清TNF-α、IFN-γ和IL-17水平均明显高于同期对照组，而IL-4水平则显著降低（P＜0.05）；经PMSC治疗后TNF-α、IFN-γ、 IL-17水平逐渐降低，IL-4水平回升，至第5周上述指标均接近对照组（P＞0.05），其中髓腔注射PMSC组TNF-α、IFN-γ、 IL-17水平较尾静脉注射PMSC组下降更为显著，而IL-4水平两组之间无明显差异.再障鼠血清造血负调控因子水平显著增高，促造血因子水平下降；PMSC能下调再障鼠造血负调控因子水平，而上调促造血因子水平，其中髓腔注射较尾静脉注射对造血负调控因子的抑制作用更为显著. |
