[Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis]

Autor:	Jian-Qiang, Deng, Bao-Qin, Liu, Ji-Feng, Cai, Wen-Hui, Li, Ren, Long, Yi-Ping, Hou
Rok vydání:	2012
Předmět:	Genotype Tandem Repeat Sequences Gene Amplification Oligonucleotides Electrophoresis Capillary Humans Reproducibility of Results DNA DNA Fingerprinting Nucleic Acid Amplification Techniques Polymerase Chain Reaction Sensitivity and Specificity DNA Primers
Zdroj:	Fa yi xue za zhi. 28(1)
ISSN:	1004-5619
Popis:	To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity.DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system.DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30 pg) by pretreatment of DOP-PCR and then detection of STR genotyping.The system established in this study is reliable and more testing sensitive for forensic trace evidence.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::6e214389d518dcb16f4490fa2cb05550 https://pubmed.ncbi.nlm.nih.gov/22435337 Zobrazit plný text záznamu