| Autor: |
A, Caterino-de-Araujo, A, Favero, E, de los Santos-Fortuna, J, Suleiman, L, Chieco-Bianchi, M L, Calabrò |
| Rok vydání: |
2000 |
| Předmět: |
|
| Zdroj: |
AIDS research and human retroviruses. 16(8) |
| ISSN: |
0889-2229 |
| Popis: |
A serological survey for HTLV infection identified an AIDS patient with HTLV-I/HTLV-II dual seroreactivity. Two further sequential blood samples were collected (samples A and B) for PCR, restriction fragment length polymorphism (RFLP), and sequence analyses of HTLV-I and HTLV-II strains. PCR analyses confirmed dual infection in both samples. Restriction digests of the env region amplified from sample A showed the presence of an HTLV-IIa subtype; the HTLV-II provirus was found to be defective in the pol and env regions in the second sample from this patient. RFLP analysis of the HTLV-II LTR region of both samples confirmed this finding and identified an a5/bzl restriction type. Nucleotide sequence analyses revealed full homology in the HTLV-I env and LTR regions and in the HTLV-II LTR region between the two samples. These findings document the first case of an HTLV-I/HTLV-II coinfection that was fully confirmed and characterized by means of molecular analyses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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