Quantitative reverse transcriptase-polymerase chain reaction measured expression of MDR1 and MRP in primary breast carcinoma
| Autor: | D W, Dexter, R K, Reddy, K G, Geles, S, Bansal, M A, Myint, A, Rogakto, J C, Leighton, L J, Goldstein |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Aged 80 and over Age Factors Breast Neoplasms Middle Aged Polymerase Chain Reaction Drug Resistance Multiple Cell Line Receptors Estrogen Lymphatic Metastasis Tumor Cells Cultured Humans Regression Analysis ATP-Binding Cassette Transporters Female ATP Binding Cassette Transporter Subfamily B Member 1 Multidrug Resistance-Associated Proteins Neoplasm Metastasis Receptors Progesterone Aged Neoplasm Staging |
| Zdroj: | Clinical cancer research : an official journal of the American Association for Cancer Research. 4(6) |
| ISSN: | 1078-0432 |
| Popis: | To evaluate the clinical significance of drug resistance mechanisms in breast cancer, we examined the expression of MDR1 and MRP in primary breast carcinoma and normal adjacent tissue using a highly quantitative and reproducible reverse transcription-PCR assay. Expression of both genes was observed in all specimens examined, both tumor (n = 74) and normal adjacent tissue (n = 55). The expression of MDR1, however, was low, with the level of expression being 25 times less than the drug-resistant control cell line KB 8-5. Immunohistochemical analysis of P-glycoprotein corroborated the PCR results; only 6% (2 of 31) were positive for JSB1 staining, and 0 of 32 were positive for for UIC2. MRP expression did not exceed control cell line levels, and immunohistochemistry detected moderate levels of expression. MDR1 expression was independent of grade, stage, tumor size, nodal status, metastasis, and estrogen receptor and progesterone receptor status. There was, however, a significant correlation of MDR1 expression with age and histology. Approximately twice the expression of MDR1 was observed in the50 age group compared to the50 age group, and lobular carcinoma had 4 times the expression of MDR1 of other histological types. MRP expression was independent of all other clinical parameters. Thus, these results show that although MDR1 expression is detectable in primary breast carcinoma by PCR, this expression as measured by quantitative reverse transcriptase-PCR is extremely low. The significance of these low levels is yet to be determined. MDR1 expression was higher in50 age group and lobular carcinoma, which may contribute to poor prognosis associated with young age and lobular histology. |
| Databáze: | OpenAIRE |
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