| Autor: |
I L, Medintz, L, Berti, C A, Emrich, J, Tom, J R, Scherer, R A, Mathies |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Clinical chemistry. 47(9) |
| ISSN: |
0009-9147 |
| Popis: |
Genetic analysis of microsatellite DNA is a powerful tool used in linkage analysis, gene mapping, and clinical diagnosis. To address the expanding needs of studies of short tandem repeats (STRs), we demonstrated high-performance STR analysis on a high-throughput microchannel plate-based platform.Energy-transfer-cassette-labeled STR amplicons were separated and typed on a microfabricated 96-channel radial capillary array electrophoresis (CAE) microchannel plate system. Four-color detection was accomplished with a laser-excited confocal fluorescence rotary scanner.Multiplex STR analysis with single base-pair resolution was demonstrated on denaturing polyacrylamide gel media. The high-throughput multiplex capabilities of this genetic analysis platform were demonstrated by the simultaneous separation of STR amplicons representing 122 samples in ninety-six 5.5-cm-long channels in8 min. Sizing values obtained for these amplicons on the CAE microchannel plate were comparable to those measured on a conventional commercial CAE instrument and exhibit1% sizing variance.Energy-transfer-cassette labeling and microfabricated CAE microchannel plates allow high-performance multiplex STR analyses. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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