| Autor: |
Juan Carlos, Roa, Iván, Roa, Xabier, de Aretxabala, Angélica, Melo, Gaspar, Faría, Oscar, Tapia |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Revista medica de Chile. 132(8) |
| ISSN: |
0034-9887 |
| Popis: |
Different K-ras mutation frequencies in gallbladder cancer have been reported.To study the frequency of K-ras gene mutations in advanced gallbladder carcinoma not associated to anomalous junction of pancreatic-biliary duct (AJPBD).33 formalin fixed paraffin embedded samples of gallbladder carcinoma (30 women, age range 32-86 years) were selected. Pancreatic cancer tissue with K-ras mutations was used as control. DNA was extracted from the histological section by mean of microdissection and K-ras mutations in codon 12 were detected by polymerase chain reaction and restriction fragment length polymorphism (RFLP), using previously reported technique.Most cases were poorly differentiated adenocarcinomas. K-ras mutation was detected in 10 cases (30%) samples. No differences in K-ras mutation frequency between subserous and serous tumors were detected and no relation between histological features and the mutation was observed.K-ras mutation in codon 12 is present in 30% in our advanced gallbladder carcinomas. The study of K-ras mutation in preneoplastic lesions and early carcinonmas will be important to determine the role of this gene in the gallbladder carcinogenesis in Chile. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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