Enhanced GBX2 expression stimulates growth of human prostate cancer cells via transcriptional up-regulation of the interleukin 6 gene
| Autor: | A C, Gao, W, Lou, J T, Isaacs |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Homeodomain Proteins
Male Sequence Homology Amino Acid Transcription Genetic Interleukin-6 Prostatic Neoplasms Transfection Recombinant Proteins Gene Expression Regulation Neoplastic Mice Tumor Cells Cultured Animals Humans RNA Messenger Cloning Molecular Promoter Regions Genetic Sequence Alignment Cell Division |
| Zdroj: | Clinical cancer research : an official journal of the American Association for Cancer Research. 6(2) |
| ISSN: | 1078-0432 |
| Popis: | Previous studies demonstrated that the GBX2 homeobox gene is consistently overexpressed in cultured human prostate cancer cell lines. In this study, the human GBX2 cDNA was cloned and a quantitative reverse transcription-PCR method used to demonstrate that GBX2 mRNA expression is enhanced in approximately 70% of human prostate cancer tissues compared with normal human prostate tissues. Purified recombinant GBX2 protein binds specifically to an ATTA motif within the promoter of the interleukin 6 (IL-6) gene. Using an antisense approach, down-regulation of the expression of GBX2 correlated with decreased expression of IL-6 and an inhibition of tumorigenicity of PC3 human prostate cancer cells. In addition, in vitro growth of the antisense clones was partially restored by exogenous addition of recombinant IL-6 protein to the culture media. These data demonstrated that enhanced GBX2 expression results in a stimulation of malignant growth of prostate cancer cells and that part of this stimulation involves up-regulation in the transcription of the IL-6 gene. |
| Databáze: | OpenAIRE |
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