New silver nanoparticles induce apoptosis-like process in
| Autor: | Iurii A, Orlov, Tatiana P, Sankova, Polina S, Babich, Ilya M, Sosnin, Ekaterina Yu, Ilyechova, Demid A, Kirilenko, Pavel N, Brunkov, Gennadii L, Ataev, Alexey E, Romanov, Ludmila V, Puchkova |
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| Rok vydání: | 2016 |
| Předmět: |
Male
silver nanoparticles Silver mammalian copper metabolism Blotting Western Metal Nanoparticles Apoptosis copper status Microscopy Atomic Force Real-Time Polymerase Chain Reaction Mice Superoxide Dismutase-1 Microscopy Electron Transmission Escherichia coli Animals RNA Messenger apoptosis-like death Original Research Reverse Transcriptase Polymerase Chain Reaction Ceruloplasmin Flow Cytometry Anti-Bacterial Agents Mice Inbred C57BL Liver Crystallization bioavailability Oxidation-Reduction Copper |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Silver nanoparticles (SNPs) are new functional materials that are widely used in biomedical and industrial technologies. Two main features that make SNPs valuable are their strong antibacterial effects and low toxicity to eukaryotes. In this study, SNPs were synthesized using a modified method of reducing the metal ions to their atomic state followed by crystallization. SNPs were characterized by UV/vis spectroscopy, X-ray diffractometry, atomic force microscopy, and transmission electron microscopy (TEM). The SNPs were spherically shaped with an average linear dimension of 20 nm. In aqueous solution, the SNPs were beige-yellow in color, and they formed a black color in bacteria-rich growth media. The toxicity and bioavailability of the SNPs were tested using Escherichia coli cells and C57Bl/6 mice. Although the SNPs displayed bactericidal activity, an E. coli cell strain transformed with an expression plasmid carrying a human CTR1 ectodomain with three motives that bind Cu(II), Cu(I), and Ag(I) demonstrated increased resistance to treatment with SNPs. TEM showed that the SNPs were absorbed by the E. coli cell, and flow cytometry showed that the SNPs induced apoptosis-like death. In mice treated with SNPs (daily intraperitoneal injection of 10 μg SNPs/g body weight over 4 days), the ceruloplasmin (Cp) oxidase activity in the blood serum decreased. However, level of Cp gene expression, the relative contents of the Cp protein in the Golgi complex and in the serum did not change. Treatment with SNPs did not influence the activity of superoxide dismutase 1 in the liver and had no apparent toxic effects in mice. These findings expand the scope of application for the use of new SNPs. The data are discussed in a paradigm, in which the effects of SNPs are caused by the interference of silver ions with copper metabolism. |
| Databáze: | OpenAIRE |
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