Elevated relative fluorescence intensity of CD38 antigen expression on CD8+ T cells is a marker of poor prognosis in HIV infection: results of 6 years of follow-up
| Autor: | Z, Liu, L E, Hultin, W G, Cumberland, P, Hultin, I, Schmid, J L, Matud, R, Detels, J V, Giorgi |
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| Rok vydání: | 1996 |
| Předmět: |
Acquired Immunodeficiency Syndrome
Membrane Glycoproteins Histocompatibility Testing Fluorescent Antibody Technique HLA-DR Antigens CD8-Positive T-Lymphocytes ADP-ribosyl Cyclase 1 Antigens Differentiation Cohort Studies Antigens CD Disease Progression Humans ADP-ribosyl Cyclase N-Glycosyl Hydrolases Follow-Up Studies |
| Zdroj: | Cytometry. 26(1) |
| ISSN: | 0196-4763 |
| Popis: | Relative fluorescence intensity measurements from a flow cytometer were used to evaluate expression of CD38 and HLA-DR antigens. These molecules are associated with cellular activation and are present at increased levels on the CD8+ lymphocytes of HIV-infected subjects. In the current study, the prognostic value of mean fluorescence intensity measurements of CD38 and HLA-DR on CD8+ cells was compared to results from our previous study in which we reported prognostic value for an elevated percentage of CD8+ cells that were positive for expression of the CD38 antigen (Giorgi et al.: JAIDS 6:904-912, 1993). Using the proportional hazards model, elevated mean fluorescence intensity of CD38 expression on CD8+ cells had prognostic value for development of AIDS that was almost identical to the prognostic value of the percentage of CD8+ cells that were positive for expression of CD38. This prognostic value was in addition to that provided by the patient's CD4+ cell measurement. To our knowledge, this is the first report that a measurement of fluorescence intensity can be used as a prognostic marker in an immunodeficiency disease. Efforts are needed to establish methods that will allow widespread application of this observation in the clinical management of HIV-infected subjects. |
| Databáze: | OpenAIRE |
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