Immunomodulatory effect of a plasmid expressing CD40 ligand on DNA vaccination against human immunodeficiency virus type‐1
| Autor: | Ihata, A, Watabe, S, Sasaki, S, Shirai, A, Fukushima, J, Hamajima, K, Inoue, J, Okuda, K |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
AIDS Vaccines
Immunity Cellular Mice Inbred BALB C Membrane Glycoproteins CD40 Ligand Dose-Response Relationship Immunologic Enzyme-Linked Immunosorbent Assay HIV Infections Cytotoxicity Tests Immunologic Lymphocyte Activation Mice Immunoglobulin G Antibody Formation HIV-1 Animals Cytokines Original Article Hypersensitivity Delayed CD40 Antigens Plasmids T-Lymphocytes Cytotoxic |
| Popis: | CD40 ligand is a costimulatory molecule which acts a potent immunomodulator. We found the mice inoculated with human CD40 ligand expression plasmid (pMEhCD40L) combined with human immunodeficiency virus type-1 (HIV-1) DNA vaccine exhibited both humoral and cellular antigen-specific immunological enhancement. The expression of hCD40L induced predominantly antigen-specific immunoglobulin G (IgG) antibody response while it failed to induce mucosal IgA response. Delayed-type hypersensitivity (DTH) and cytotoxic T lymphocyte (CTL) activity were induced in a dose-dependent manner. Examination of the relative levels of the two IgG subclasses showed that co-injection of pMEhCD40L enhanced IgG2a response without suppressing IgG1 response. Similarly, the expression of pMEhCD40L enhanced not only T helper 1 (Th1)- but also Th2-type cytokine production. In conclusion, co-inoculation of pMEhCD40L with DNA vaccine was shown to be a useful way to enhance CTL responses without suppressing the humoral immune response in acquired immune deficiency syndrome (AIDS) patients. |
| Databáze: | OpenAIRE |
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