High background in ELISpot assays is associated with elevated levels of immune activation in HIV-1-seronegative individuals in Nairobi

Autor:	Amy Y, Liu, Stephen C, De Rosa, Brandon L, Guthrie, Robert Y, Choi, Rose, Kerubo-Bosire, Barbra A, Richardson, James, Kiarie, Carey, Farquhar, Barbara, Lohman-Payne
Rok vydání:	2018
Předmět:	Adult CD4-Positive T-Lymphocytes Male Enzyme-Linked Immunospot Assay Membrane Glycoproteins IGRA HLA-DR Antigens CD8-Positive T-Lymphocytes Lymphocyte Activation ADP-ribosyl Cyclase 1 Kenya Interferon-gamma Young Adult HIV Seronegativity HIV-1 HIV‐seronegative Humans Female Flow cytometry Cities Original Research
Zdroj:	Immunity, Inflammation and Disease
ISSN:	2050-4527
Popis:	Introduction Spontaneous interferon‐γ (IFNγ) released detected by enzyme‐linked immunospot (ELISpot) assays may be a biological phenomenon. Markers of immune activation levels were assessed as correlates of high background among individuals in Kenya. Methods Couples concordantly seronegative for HIV‐1 were enrolled. IFN‐γ ELISpot assays were conducted and negative control wells were categorized as having either high or low background (≥50 and
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::6c1aa43f56b07d6d1ad60356e6d52f31 https://pubmed.ncbi.nlm.nih.gov/29974672 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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