Expression of a synthetic antifreeze protein in potato reduces electrolyte release at freezing temperatures

Autor:	J G, Wallis, H, Wang, D J, Guerra
Rok vydání:	1998
Předmět:	Base Sequence Recombinant Fusion Proteins Molecular Sequence Data Antibodies Plant Leaves Electrolytes Transformation Genetic Antifreeze Proteins Freezing Genes Synthetic Amino Acid Sequence Phytohemagglutinins Plant Lectins Glycoproteins Plant Proteins Solanum tuberosum
Zdroj:	Plant molecular biology. 35(3)
ISSN:	0167-4412
Popis:	A synthetic antifreeze protein gene was expressed in plants and reduced electrolyte leakage from the leaves at freezing temperatures. The synthetic AFP was expressed as a fusion to a signal peptide, directing it to the extracytoplasmic space where ice crystallization first occurs. The gene was introduced to Solanum tuberosum L. cv. Russet Burbank by Agrobacterium-mediated transformation. Transformants were identified by PCR screening and expression of the introduced protein was verified by immunoblot. Electrolyte-release analysis of transgenic plant leaves established a correlation between the level of transgenic protein expression and degree of tolerance to freezing. This is the first identification of a phenotype associated with antifreeze protein expression in plant tissue.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::6bf5315b98d6e33b38e96261ce11b01f https://pubmed.ncbi.nlm.nih.gov/9349256 Zobrazit plný text záznamu Full text from SpringerLink