Autor: |
Alexander, Staruschenko, Rachell E, Booth, Oleh, Pochynyuk, James D, Stockand, Qiusheng, Tong |
Rok vydání: |
2006 |
Předmět: |
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Zdroj: |
Methods in molecular biology (Clifton, N.J.). 337 |
ISSN: |
1064-3745 |
Popis: |
Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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