Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system

Autor: Alexander, Staruschenko, Rachell E, Booth, Oleh, Pochynyuk, James D, Stockand, Qiusheng, Tong
Rok vydání: 2006
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 337
ISSN: 1064-3745
Popis: Probing ion channel structure-function and regulation in native tissue can, in some instances, be experimentally challenging or impractical. To facilitate discovery and increase experimental flexibility, our laboratory routinely reconstitutes recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells.
Databáze: OpenAIRE