Prostate short-chain dehydrogenase reductase 1 (PSDR1): a new member of the short-chain steroid dehydrogenase/reductase family highly expressed in normal and neoplastic prostate epithelium
| Autor: | B, Lin, J T, White, C, Ferguson, S, Wang, R, Vessella, R, Bumgarner, L D, True, L, Hood, P S, Nelson |
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| Rok vydání: | 2001 |
| Předmět: |
Male
DNA Complementary Base Sequence Sequence Homology Amino Acid Gene Expression Profiling Molecular Sequence Data Prostate Chromosome Mapping Prostatic Neoplasms DNA Neoplasm Epithelium Gene Expression Regulation Neoplastic Gene Expression Regulation Androgens Tumor Cells Cultured Humans Amino Acid Sequence RNA Messenger Cloning Molecular Oxidoreductases Promoter Regions Genetic Oligonucleotide Array Sequence Analysis |
| Zdroj: | Cancer research. 61(4) |
| ISSN: | 0008-5472 |
| Popis: | Genes regulated by androgenic hormones are of critical importance for the normal physiological function of the human prostate gland, and they contribute to the development and progression of prostate carcinoma. We used cDNA microarrays comprised of prostate-derived cDNAs to profile transcripts regulated by androgens in prostate cancer cells. This study identified a novel gene that we have designated prostate short-chain dehydrogenase/reductase 1 (PSDR1), that exhibits increased expression on exposure to androgens in the LNCaP prostate cancer cell line. Northern analysis demonstrated that PSDR1 is highly expressed in the prostate gland relative to other normal human tissues. The PSDR1 cDNA and putative protein exhibit homology to the family of short-chain dehydrogenase/reductase enzymes and thus identify a new member of this family. Cloning and analysis of the putative PSDR1 promoter region identified a potential androgen-response element. We used a radiation-hybrid panel to map the PSDR1 gene to chromosome 14q23-24.3. In situ hybridization localizes PSDR1 expression to normal and neoplastic prostate epithelium. These results identify a new gene involved in the androgen receptor-regulated gene network of the human prostate that may play a role in the pathogenesis of prostate carcinoma. |
| Databáze: | OpenAIRE |
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