| Autor: |
Y, Cheng, H-Y, Xiong, Y-M, Li, H-R, Zuo, Y, Liu, G-L, Liao |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
European review for medical and pharmacological sciences. 25(14) |
| ISSN: |
2284-0729 |
| Popis: |
Long noncoding RNA (lncRNA) was found to play crucial roles in regulating cancer progression. HOXA11 antisense RNA (HOXA11-AS) was reported to serve an oncogenic lncRNA in cancers but its role in prostate cancer (PCa) remains to be explored.Expression levels of HOXA11-AS in PCa tissues and cells were analyzed with quantitative Real-Time PCR method. MTT assay, colony formation assay, transwell invasion assay, and flow cytometry assay were conducted to explore the biological roles of HOXA11-AS in PCa. Rescue experiments were conducted to investigate mechanisms of HOXA11-AS in regulating PCa progression.We revealed that HOXA11-AS was upregulated in PCa. Silencing of HOXA11-AS significantly inhibited PCa cell proliferation, colony formation, invasion, and promoted apoptosis in vitro. On the contrary, forcing of HOXA11-AS expression caused opposite effects on cancer cell behaviors. Furthermore, we showed that HOXA11-AS1 serves as a competing endogenous RNA (ceRNA) to regulate Jupiter microtubule associated homolog 1 (JPT1) via sponging microRNA-24-3p (miR-24-3p). Functionally, the overexpression of miR-24-3p or knockdown of JPT1 could partially reverse the effects of HOXA11-AS overexpression on PCa cell behaviors.This newly identified HOXA11-AS/miR-24-3p/JPT1 axis may provide novel angle for the better control of PCa. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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