[Elaboration of the polymerase chain reaction system for subtype specific differentiation of influenza A virus strains]

Autor:	K V, Tarasov, S Iu, Tsvirkunova, T A, Bekhtereva
Rok vydání:	1995
Předmět:	Influenza A virus DNA Viral Hemagglutinins Viral RNA Viral Templates Genetic Polymerase Chain Reaction Plasmids
Zdroj:	Vestnik Rossiiskoi akademii meditsinskikh nauk. (9)
ISSN:	0869-6047
Popis:	New subtype specific primers for hemagglutinin (H1, H2, H3) of influenza A viruses were selected. The specificity of the primers' performance was tested in a model experiment which used cDNA synthesized on the vRNA from the collection of influenza A viruses (H1, H2, H3 subtypes) as templates. The sensibility of the PCR system was determined on DNA plasmids- and RNA templates (with preliminary reverse transcription) and amounted to 10(2) and 10(5)-10(6) virion particles, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::6b5b8c1d2aa911ed999db65f2a0447a8 https://pubmed.ncbi.nlm.nih.gov/7580407 Zobrazit plný text záznamu