| Autor: |
R F, Williams, T L, Sims, L, Tracey, A L, Myers, C Y C, Ng, H, Poppleton, A C, Nathwani, A M, Davidoff |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Anticancer research. 30(9) |
| ISSN: |
1791-7530 |
| Popis: |
The vascular niche necessary for cancer stem cell maintenance is a potential target for cancer therapy.Human glioma xenografts were treated with IFN-β delivered systemically via a liver-targeted, adeno-associated viral vector. The vascular niche was examined with immunofluorescence for glioma stem cells, endothelial cells, and perivascular cells.Although IFN-β was not directly toxic to glioma stem cells in vitro, IFN-β decreased tumor size and the number of stem cells recovered in both heterotopic and orthotopic models. Treatment with IFN-β increased perivascular cells investing the tumor vasculature (6-fold) distancing stem cells from endothelial cells. Additionally, vascular smooth muscle cells co-cultured between stem cells and endothelial cells decreased stem cell recovery.Continuous delivery of IFN-β decreased the number of stem cells in glioma xenografts by disrupting the vascular niche through an increase in perivascular cells, which created a barrier between the glioma stem cells and the endothelial cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
