Clinical and methodological factors affecting non-transferrin-bound iron values using a novel fluorescent bead assay

Autor: Maciej W, Garbowski, Yongmin, Ma, Suthat, Fucharoen, Somdet, Srichairatanakool, Robert, Hider, John B, Porter
Rok vydání: 2015
Předmět:
Nitrilotriacetic Acid
NTBI
nontransferrin-bound iron
NRBC
nucleated red blood cells
Iron
SF
serum ferritin
Iron Chelating Agents
LIC
liver iron content
Fluorescence
DFP
deferiprone
ULN
upper limit of normal
Tf
transferrin
Humans
CIC
cardiac iron content
f.c.
final concentration
CSA
congenital sideroblastic anemia
DBA
Diamodn-Blackfan anemia
IQR
interquartile range
DFO
deferoxamine
AlAT
alanine-aminotransferase
TfSat
transferrin saturation
DCI
directly chelatable iron
Transferrin
ELISA
enzyme-linked immunosorbent assay
Microspheres
UV
ultraviolet
CI
confidence interval
LPI
labile plasma iron
sTfR
soluble transferrin receptors
CFBs
control fluorescent beads
MDS
myelodysplastic syndrome
Regression Analysis
MOPS
3-(N-morpholino)propanesulfonic acid
Biological Assay
Original Article
AAS
atomic absorption standard
FBC
full blood count
NTA
nitrilotriacetic acid
SD
standard deviation
DFX
deferasirox
Zdroj: Translational Research
ISSN: 1878-1810
Popis: Nontransferrin-bound iron (NTBI) is a heterogeneously speciated plasma iron, typically detectable when transferrin saturation (TfSat) exceeds 75%. Here, we examine factors affecting NTBI levels by a recently discovered direct chelator-based (CP851) fluorescent bead-linked flow-cytometric assay (bead-NTBI), compared with the established indirect nitrilotriacetate (NTA) assay in 122 iron-overloaded patients, including 64 on recent iron chelation therapy and 13 healthy volunteers. Both methods correlated (r = 0.57, P
Databáze: OpenAIRE
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