Induction of anti-progesterone immunity and pregnancy blocking by anti-progesterone anti-idiotypes. Variable efficacy of polyclonal Ab2 antibodies directed against a panel of closely related Ab1 antibodies
| Autor: | M W, Wang, M J, Sims, P R, Symington, A S, Humphreys, M J, Taussig |
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| Rok vydání: | 1991 |
| Předmět: |
Mice
Inbred BALB C Base Sequence Molecular Sequence Data Autoimmunity Antibodies Anti-Idiotypic Mice Fertility Immunoglobulin Idiotypes Antibody Specificity Pregnancy Animals Female Immunoglobulin Light Chains Amino Acid Sequence Contraception Immunologic Immunoglobulin Heavy Chains Progesterone Research Article |
| Zdroj: | Immunology. 73(3) |
| ISSN: | 0019-2805 |
| Popis: | Polyclonal rabbit anti-idiotypes (Ab2) have been raised against three mouse monoclonal antiprogesterone Ab1 antibodies (DB3, 11/32, 11/64) closely related in VH and VL sequences. The anti-idiotypes were characterized for specificity and used to immunize groups of female mice. The latter responded with production of anti-progesterone (Ab3) antibodies, confirming the ability of anti-idiotypes to mimic the immunogenicity of a steroid. The response to one of the anti-idiotypic reagents (anti-DB3-id) was 5-10 times stronger than those to the others, despite close sequence homology between the idiotypes. Moreover, immunization with anti-DB3-id led to a reduction in fertility rate from 90% (control) to 30%, whereas immunization with the other anti-idiotypes was without effect. Sequence and structural comparisons suggest that residues associated with VH CDR3 and VL CDR3 may have a key role in determining the efficiency of anti-idiotypic immunization against progesterone. The variability in outcome of using anti-idiotypic reagents against a defined panel of related antibodies is relevant to the use of anti-idiotypes as surrogate antigens. |
| Databáze: | OpenAIRE |
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