| Autor: |
Koen D, Reesink, Tim, Hendrikx, Patrick J, van Gorp, Arnold P, Hoeks, Ronit, Shiri-Sverdlov |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine. 37(4) |
| ISSN: |
1550-9613 |
| Popis: |
To investigate the utility of ultrasonic (US) perfluorohexane (PFH)-loaded monocyte imaging for detection of liver inflammation in fatty liver disease.C57Bl6 mice were injected intraperitoneally with tumor necrosis factor α and assessed by US PFH-loaded monocyte imaging 3 hours later. Echogenic monocytes were injected intravenously, leading to a transient increase in liver tissue intensity on a US perfusion scan. The contrast wash-out time constant was hypothesized to reflect the degree of inflammation. Next, we evaluated US PFH-loaded monocyte imaging in LdlrTumor necrosis factor α-injected mice showed a reduced wash-out time constant (mean ± SEM, 0.013 ± 0.003; n = 8) compared to controls (0.054 ± 0.009; n = 7; P = .0006), indicative of increased inflammatory adhesion molecule expression on the endothelium. The LdlrOur results indicate that US PFH-loaded monocyte imaging is able to detect vascularly expressed inflammatory adhesion molecules in the mouse liver on direct endothelial stimulation. However, in our mouse model of early developing nonalcoholic steatohepatitis, we did not detect inflammation by this method, which may suggest that the time-dependent relationship between parenchymal and endothelial inflammation remains a fundamental issue to be addressed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Plný text