| Autor: |
Yan, Qin, Li-ya, He, Yong, Chen, Wen-yi, Wang, Xue-hua, Zhao, Mian-yun, Wu |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 28(1) |
| ISSN: |
1007-8738 |
| Popis: |
Quercetin affects the expressions of leptin and its receptor in human gastric cancer MGC-803 cells and JAK-STAT pathway.The cultured MGC-803 cells were divided into three groups:the cultured cells without quercetin, and Quercetin group: the cultured cells with quercetin(40 μmol/L), and AG490group: the cultured cells with AG490(40 μmol/L)The expressions of Leptin, Leptin receptor and P-STAT3 were detected in protein level by immunocytochemical and Western bloting method respectively. The expressions of Leptin, Leptin receptor were detected in mRNA level by RT-PCR method. MGC-803 cell cycle was arrest by flow cytometry (FCM); MGC-803 cell apoptosis ratio by apoptotic marker An-necxinV.The protein expression of Leptin, Leptin receptor, P-STAT3 and the the mRNA expression of Leptin and Leptin receptor were significantly increased (P0.05), compared with the control group.There was the rectilinear correlation relationship not only between Leptin and P-STAT3 protein(r=0.741, P0.05) but also between Leptin receptor and P-STAT3 protein(r=0.693, P0.05). FCM analysis showed that quercetin arrested MGC-803 cells at the G2/M phase, The ratio of apoptotic and necrosic cells increased with added quercetin concentration.Quercetin could inhibit the Proliferation of MGC-803 cells. It is probably relevant to the down-regulation the expressions of Leptin and Leptin receptor protein, Leptin mRNA and Leptin receptor mRNA by JAK-STAT pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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