A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the Great Artesian Basin of Australia

Autor:	C, Wynter, B K, Patel, P, Bain, J, de Jersey, S, Hamilton, P A, Inkerman
Rok vydání:	1996
Předmět:	Hot Temperature Molecular Sequence Data Australia Fresh Water Dextranase Bacteria Anaerobic RNA Bacterial Genes Bacterial RNA Ribosomal 16S Enzyme Stability Carbohydrate Metabolism Water Microbiology Gram-Positive Asporogenous Rods Irregular Half-Life
Zdroj:	FEMS microbiology letters. 140(2-3)
ISSN:	0378-1097
Popis:	A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 degrees C and a pH optimum between 5 and 6. The half life was approximately 6.5 h at 75 degrees C and 2 h at 80 degrees C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB11Ad was a member of the genus Thermoanaerobacter.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::693928d99b23e3bce207e42d9cefaa08 https://pubmed.ncbi.nlm.nih.gov/8764490 Zobrazit plný text záznamu Plný text