| Autor: |
Nazan, Atalan, Hande, Karagedik, Leyla, Acar, Selim, Isbir, Seda Gulec, Yilmaz, Arzu, Ergen, Turgay, Isbir |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
In vivo (Athens, Greece). 30(5) |
| ISSN: |
1791-7549 |
| Popis: |
To analyze the effect of TLR-9 (-1486 TC) and TLR-9 (CT) gene polymorphisms both at TLR-9 levels and together with their sepsis parameters. In this regard, the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used in order to detect TLR-9 gene polymorphisms, whereas the ELISA technique was used to analyze TLR-9 serum levels in 80 sepsis patients and 100 healthy individuals.The study group consisted of 80 patients with a diagnosis of sepsis and 100 healthy individuals. TLR-9 CT polymorphism was identified by PCR-RFLP.There was no substantial difference observed between sepsis and control groups in terms of TLR-9 (-1486 TC) and TLR-9 (CT) genotype and allele distribution. When serum TLR-9 levels were compared to TLR-9 (-1486 TC) and TLR-9 (CT) genotype and allele distribution, there was a statistically substantial decrease in TLR-9 serum levels of both TLR-9 (-1486 TC) TT and TLR-9 (CT) TT individuals in the sepsis group (p=0.011 and p=0.036, respectively).There is no relation between sepsis and both TLR-9 (CT) and TLR-9(-1486 TC) polymorphisms; however, there is a relation between sepsis and decreased serum TLR-9 levels of both TLR-9 (-1486 TC) TT and TLR-9 (CT) TT polymorphisms due to sepsis-associated immunosuppression. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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