Activation of the human delta-globin gene promoter in primary adult erythroid cells

Autor:	D C, Tang, G P, Rodgers
Rok vydání:	1998
Předmět:	Adult DNA-Binding Proteins Mutagenesis Insertional Erythroblasts CCAAT-Enhancer-Binding Proteins Kruppel-Like Transcription Factors Gene Expression Humans Promoter Regions Genetic Globins Transcription Factors
Zdroj:	British journal of haematology. 103(3)
ISSN:	0007-1048
Popis:	Restoration of the CCAAT box or insertion of an erythroid Krüppel-like factor (EKLF) binding site in the delta promoter activates its expression in several erythroid cell lines. We extended these studies using a novel primary human adult erythroid cell (hAEC) system to investigate these effects at the late erythroblast stage. Restoration of the CCAAT box at -70 bp, or insertion of an EKLF binding site at -85 bp or -95 bp in the promoter significantly increased delta globin gene expression in hAEC. Our results demonstrate that the altered CCAAT box (CCAAC) and the lack of an EKLF binding site in delta-globin contribute to its low level of expression in the hAEC model as well.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::68c9a1938ade164a0cf4c3cbf1f68dc7 https://pubmed.ncbi.nlm.nih.gov/9858241 Zobrazit plný text záznamu Plný text