| Autor: |
Y W, Kim, H, Wang, I, Sures, R, Lammers, K J, Martell, A, Ullrich |
| Rok vydání: |
1996 |
| Předmět: |
|
| Zdroj: |
Oncogene. 13(10) |
| ISSN: |
0950-9232 |
| Popis: |
Using a polymerase chain reaction (PCR) amplification strategy, we identified a novel protein tyrosine phosphatase (PTPase) designated Brain Derived Phosphatase (BDP1). The full length sequence encoded an open reading frame of 459 amino acids with no transmembrane domain and had a calculated molecular weight of 50 kDa. The predicted amino acid sequence contained a PEST motif and accordingly, BDP1 shared the greatest homology with members of the PTP-PEST family. When transiently expressed in 293 cells BDP1 hydrolyzed p-Nitrophenylphosphate, confirming it as a functional protein tyrosine phosphatase. Northern blot analysis indicated that BDP1 was expressed not only in brain, but also in colon and several different tumor-derived cell lines. Furthermore, BDP1 was found to differentially dephosphorylate autophosphorylated tyrosine kinases which are known to be overexpressed in tumor tissues. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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