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To observe the effects of down-regulation of long non-coding RNA HOX antisense intergenic RNA myeloid 1 (LncRNA-HOTAIRM1) to the proliferation and apoptosis of Jurkat in human leukemia T lymphocytes, and explore its mechanism.Jurkat cells were cultured in vitro and randomly divided into control group, HOTAIRM1 siRNA-NC group and HOTAIRM1 siRNA group; the expressions of LncRNA-HOTAIRM1 mRNA, KIT receptor tyrosine kinase (KIT) mRNA and serine threonine kinase (AKT) mRNA in Jurkat cells were detected by real-time fluorescence quantification (RT-qPCR); the proliferation of Jurkat cells in each groups was detected by CCK-8 method; the apoptosis of Jurkat cells in each groups was detected by Annexin V-FITC/PI double staining; the expressions of KIT, AKT, p-KIT, p-AKT, B-lymphoma-2 gene (BCL-2) and Caspase-3 were detected by Western blot.Compared with the cells in the control group and HOTAIRM1 siRNA-NC group, the expression level of LncRNA-HOTAIRM1 mRNA, cell survival rate, expression levels of KIT mRNA, AKT mRNA, p-KIT, p-AKT and BCL-2 proteins in Jurkat cells in HOTAIRM1 siRNA group were significantly lower (P0.05), while the expression level of Cleared Caspase-3 protein and Jurkat cell apoptosis rate were significantly higher (P0.05).LncRNA-HOTAIRM1 may inhibit Jurkat cell proliferation and induce apoptosis through KIT/AKT signaling pathway.LncRNA-HOTAIRM1下调对Jurkat细胞增殖、 凋亡及KIT/AKT通路的影响.观察长链非编码-HOX反义基因间RNA髓样1（LncRNA-HOTAIRM1）下调对人白血病T淋巴细胞Jurkat增殖及凋亡的影响，并初步探讨其作用机理。.体外培养Jurkat细胞，随机分为对照组、HOTAIRM1 siRNA-NC组、HOTAIRM1 siRNA组；利用实时荧光定量PCR法检测转染后各组Jurkat细胞LncRNA-HOTAIRM1 mRNA、KIT受体酪氨酸激酶（KIT） mRNA、丝氨酸-苏氨酸激酶（AKT） mRNA的表达情况；采用CCK-8法检测各组Jurkat细胞的增殖情况；利用Annexin V-FITC/PI双染法检测各组Jurkat细胞的凋亡情况；采用蛋白印迹分析法检测KIT、AKT、p-KIT、p-AKT、B淋巴细胞瘤-2基因（BCL-2）及半胱氨酸天冬氨酸酶3（Caspase-3）蛋白表达的情况。.与对照组和HOTAIRM1 siRNA-NC组相比，HOTAIRM1 siRNA组Jurkat细胞LncRNA-HOTAIRM1 mRNA表达水平、细胞存活率、KIT mRNA、AKT mRNA、p-KIT、p-AKT及BCL-2蛋白表达水平均显著降低（P0.05），Cleared Caspase-3蛋白表达水平、Jurkat细胞凋亡率显著升高（P0.05）。.LncRNA-HOTAIRM1可能通过KIT/AKT信号通路，抑制Jurkat细胞增殖并诱导其凋亡。. |
