Exosomal p38 Mitogen-activated Protein Kinase Promotes Tumour Repopulation in

Autor:	Mami, Osawa, Yasunobu, Matsuda, Jun, Sakata, Toshifumi, Wakai
Rok vydání:	2021
Předmět:	Antineoplastic Agents Exosomes Models Biological p38 Mitogen-Activated Protein Kinases Oxidative Stress Bile Duct Neoplasms Cell Line Tumor Culture Media Conditioned Mutation Humans Gene Silencing Tumor Suppressor Protein p53 Protein Kinase Inhibitors Cell Proliferation Signal Transduction
Zdroj:	Anticancer research. 42(2)
ISSN:	1791-7530
Popis:	Tumour repopulation is a major obstacle for successful cancer treatment. This study investigated whether anticancer agents contribute to tumour repopulation in TP53-mutated bile duct cancer cells.TP53-mutated HuCCT1 and HuH28 cells were exposed to anticancer agents, and recipient cells were exposed to their conditioned media or exosomes. The effect of inhibitors and siRNA-mediated gene silencing of p38 mitogen-activated protein kinase (MAPK) and of TP53 was analyzed by cell proliferation assays and western blotting.Conditioned media from genotoxic agent-treated cells promoted proliferation of recipient cells (p0.05), and this effect was abrogated by exosome inhibitors. Exosomes from gemcitabine- or cisplatin-treated cells increased cell proliferation by 1.6- to 2.2-fold (p0.05) through p38 MAPK signalling. These effects of exosomes were inhibited by inhibition/silencing of p38 MAPK but not by TP53 silencing.Exosomal p38 MAPK plays a pivotal role in tumour repopulation in a TP53-independent manner.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::67f0080395fafeed13865f54d3db54f9 https://pubmed.ncbi.nlm.nih.gov/35093873 Zobrazit plný text záznamu