| Autor: |
M A, Shliankevich, D S, Pirtskhalaishvili, T B, Prigozhina, O B, Drize, V S, Shapot |
| Rok vydání: |
1975 |
| Předmět: |
|
| Zdroj: |
Biulleten' eksperimental'noi biologii i meditsiny. 79(1) |
| ISSN: |
0365-9615 |
| Popis: |
SV40 T-antigens were isolated from an extract of golden hamster tumours by precipitation with ammonium sulphate with subsequent fractionation on DEAE cellulose. The degree of purification of the preparation proved to be about 100-fold; it, however, contained an admixture of several cell proteins. Treatment of the DNA of the calf thymus with the T-antigen preparation in the presence of magnesium ions decreased the viscosity of the DNA solution during the first hour of incubation. T-antigen inactivated by heating, and also a fraction of normal hamster tissues analogous to it produced no such effect. In case of centrifugation in the saccharose gradient the constant of DNA sedimentation fell after the treatment with T-antigen from 285 to 165, this corresponding to about4--5-fold reduction of molecular weight of the DNA. The data obtained indicated that the partially purified T-antigen preparation possessed endonuclease activity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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