| Autor: |
A A, Sereĭskaia, T V, Osadchuk, A I, Korneliuk, S B, Serebrianyĭ, S A, Atepalikhina |
| Rok vydání: |
1986 |
| Předmět: |
|
| Zdroj: |
Biokhimiia (Moscow, Russia). 51(10) |
| ISSN: |
0320-9725 |
| Popis: |
It was shown that selective hydrolysis of the disulfide bridge between the A- and B-chains of human thrombin in the absence of denaturating agents decrease its proteolytic (e.g., fibrinogen-binding), esterase and amidase activities. Both chains remain bound by non-covalent interactions. A preparation of partially reduced thrombin was obtained and its kinetic parameters were determined. The experimental results suggest that the S-S bond connecting the A- and B-chains of thrombin is involved in the stabilization of the enzyme active center. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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