Hemoglobin A2 quantification by capillary zone electrophoresis

Autor:	Z K, Shihabi, M E, Hinsdale, H K, Daugherty
Rok vydání:	2000
Předmět:	Erythrocytes Hemoglobins Abnormal Electrophoresis Capillary Humans Reproducibility of Results Indicators and Reagents Hemoglobin A2 Sickle Cell Trait
Zdroj:	Electrophoresis. 21(4)
ISSN:	0173-0835
Popis:	Hemoglobin A2 (HbA2) comprises about 2.2% of the total hemoglobin in the erythrocytes. The separation and quantitation of this minor hemoglobin by capillary electrophoresis (CE) using an arginine Tris buffer is described. Some of the variables affecting the accuracy and precision of HbA2 quantification are investigated. Furthermore, the quantification of this hemoglobin by CE is compared to that of a microcolumn chromatography method. The CE method is better suited than the microcolumn method for measuring HbA2 in the sickle cell trait.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::66afc028516cd591e56a35ce82550375 https://pubmed.ncbi.nlm.nih.gov/10733216 Zobrazit plný text záznamu Plný text