Protein recovery from the all-agarose ProSieve gel system

Autor: J H, Morgan, F P, Curtis, S, Nochumson
Rok vydání: 1991
Předmět:
Zdroj: BioTechniques. 11(2)
ISSN: 0736-6205
Popis: Electrophoresis in polyacrylamide gels is one of the most powerful tools used for the analysis of proteins. However, this technique is not widely used for protein purification for a variety of reasons such as the following: less than quantitative recoveries; involved, time-consuming methodologies; and impurities in the protein preparations from gel-polymerization by-products that can modify the proteins and interfere with subsequent experiments. As an alternative, we have developed a simple and quantitative recovery procedure for proteins separated by electrophoresis in the all-agarose ProSieve gel system. Using this procedure, greater than 90% of each protein examined was recovered, and these proteins were unaffected by the recovery procedure.
Databáze: OpenAIRE