| Autor: |
A R, Satoskar, S, Rodig, S R, Telford, A A, Satoskar, S K, Ghosh, F, von Lichtenberg, J R, David |
| Rok vydání: |
2000 |
| Předmět: |
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| Zdroj: |
European journal of immunology. 30(3) |
| ISSN: |
0014-2980 |
| Popis: |
To determine the in vivo role of IL-12 in the development of protective immunity in visceral leishmaniasis caused by Leishmania donovani, we examined the course of L. donovani infection in IL-12-deficient C57BL/6 (IL-12-/-) mice. IL-12-/- mice displayed significantly higher parasite burdens in their livers and spleens than wild-type C57BL/6 mice throughout the course of infection. Despite high parasite burdens, the onset of hepatosplenomegaly was significantly delayed in L. donovani-infected IL-12-/-. Moreover, livers and spleens from IL-12-/- mice displayed significantly less inflammation and poorly formed granulomatous lesions than those from IL-12+/+ mice throughout the course of infection. Antigen-stimulated splenocytes from IL-12-/- mice produced significantly less IFN-gamma but more IL-4 than IL-12+/+ mice. These findings indicate that although endogenous IL-12 is critical for the development of protective immunity to L. donovani, it is also responsible for inducing the significant immunopathology associated with visceral leishmaniasis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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